Evaluation of the efficacy of LAMP-based SARS-CoV-2 detection with simple RNA extraction from nasopharyngeal swabs: A prospective observational study

Karino, Masaki and Harada, Mizuki and Yamada, Chihiro and Fukuoka, Kyoko and Sugo, Megumi and Hanada, Hiroyuki and Masuda, Daisaku and Adachi, Shingo and Nakao, Shota and Seki, Masayuki and Yamato, Masaya and Yamashita, Shizuya and Takano, Toru and Kalendar, Ruslan (2021) Evaluation of the efficacy of LAMP-based SARS-CoV-2 detection with simple RNA extraction from nasopharyngeal swabs: A prospective observational study. PLOS ONE, 16 (12). e0260732. ISSN 1932-6203

[thumbnail of journal.pone.0260732.pdf] Text
journal.pone.0260732.pdf - Published Version

Download (723kB)

Abstract

The Loopamp SARS-CoV-2 Detection Kit is used for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Loop-mediated isothermal amplification (LAMP) is based on a measurement principle that can be used with a relatively simple device. Detection using this kit requires viral RNA extraction from samples with the QIAGEN QIAamp Viral Mini Kit (QIAGEN extraction) or the Loopamp Viral RNA Extraction Kit (Eiken extraction), which are recommended by the manufacturer. However, the efficacy of LAMP-based SARS-CoV-2 detection using these extraction methods has not been compared. In this study, we aimed to compare the results of genome extraction and detection from nasopharyngeal swab samples using the QIAGEN and Eiken extraction kits. The present study involved patients who presented to the Rinku General Medical Center with suspected COVID-19 (25 positive and 26 negative cases). A comparison of the results obtained using each extraction method with those obtained via PCR showed that the positive, negative, and overall concordance rates between QIAGEN extraction and PCR were 96.0% (24/25 samples), 100% (26/26), and 98.0% (50/51; κ = 0.96, 95% CI = 0.69–1.00), respectively. Results with Eiken extraction were also favorable, with positive, negative, and overall concordance rates of 88.0% (22/25), 100% (26/26), and 94.1% (48/51; κ = 0.88, 95% CI = 0.61–1.00), respectively. Favorable results were obtained using both QIAGEN and Eiken extraction kits. Since Eiken extraction can be completed in a few minutes, it enables prompt and reliable testing for SARS-CoV-2 detection.

Item Type: Article
Subjects: Universal Eprints > Biological Science
Depositing User: Managing Editor
Date Deposited: 04 Jan 2023 05:14
Last Modified: 18 May 2024 06:55
URI: http://journal.article2publish.com/id/eprint/916

Actions (login required)

View Item
View Item