IN-VITRO REGENERATION AND CALLUS FORMATION FROM LEAVES OF A MEDICINAL PLANT, “SPONDIAS MANGIFERA” (L.)

Callus proliferation was studied on leaf of explant of Spondias mangifera L. cultured on Murashige and Skoog's medium (MS) supplemented with auxin (2, 4D, IBA, IAA and NAA) and cytokinin (BAP) alone or in combination. Most suitable medium noted for shoot formation from leaves was BAP (2.5 ppm) in MS medium. Shoot developed was rooted the best in MS medium with 0.5 ppm NAA. Culture kept under various conditions of light and temperature to find the suitable physical condition for the best growth.