The Addition Effect of Sea Cucumber Extract (Stichopodidae) on the Quality of Snakehead Fish oil (C. striata) during Storage

Snakehead fish (Channa striata) is one of the freshwater fish consumptions that has high economic value so that many people like it. Processing snakehead fish into fish oil can be an alternative to product diversification. Fish oil is an oil that contains high unsaturated fatty acids that are easily oxidized. Adding antioxidants to the oil can suppress oxidation so it is necessary to add antioxidant agents derived from natural ingredients such as sea cucumber extract (Stichopodidae). The purpose of this study was to examine the effectiveness of sea cucumber extract antioxidants with the best concentration on changes in the quality of snakehead fish oil obtained from previous research during storage. The material used in this study was sea cucumber extract and snakehead fish oil. The study was carried out using experimental laboratories method using Split Plot in Time design where the difference in adding sea cucumber extract as the main plot and storage duration as a sub-plot for Thiobarbituric acid (TBA), Peroxide Value (PV), and Free Fatty Acid (FFA) tests. The treatment of this study was the addition of sea cucumber extract (0% as a control; 0.1%; 0.2%; 0.3%) with 2 storage points (0 and 5 days) for 3 repeats. Making sea cucumber extract extraction method using 96% ethanol. Parametric data were analyzed with Analysis of Variance tests (ANOVA) and Honest Real Difference (HRD) while non-parametric data were analyzed with Kruskal Wallis and Mann-Whitney. The results of preliminary research obtained sea cucumber extract ration with 96% ethanol solvent of 16.1%, % inhibition of 80.30% and antioxidant activity of IC50 of 80.875 ppm (strong). The results of the main study obtained TBA values ranging from 0.07 to 0.27 mg. malonaldehyde/kg, PV range from 0.13 to 5.31 mEq/kg, and Free Fatty Acid (FFA) values range from 0.39 to 1.54%. Based on the results of the study showed that the interaction between the concentration of sea cucumber extract and the duration of storage had a real effect (p < 0.05). 0% sea cucumber extract produced the lowest TBA sapling, which was 0.07±0.05 mg. malonaldehyde/kg, 0.1% yielded the lowest peroxide number at 3.40±1.25 mEq/kg, and 0.2% yielded the lowest FFA number of 0.58±0.00% on day 5. The results suggest that ethanolic extract of sea cucumber does not act as an efficient antioxidant agent to prevent fish oil degradation.