Variation in Crude Protein Content among Recombinant Inbred Lines of Rice (Oryza sativa L.)

Rice, Oryza sativa L., is the world's most important staple crop, feeding more than half of the world's population, protein plays important role in human nutrition. Protein is the second most abundant storage matter in rice grain. Protein, crucial for growth, antibody production, and immunity, ranks as the second most abundant storage component in rice grains. Various factors, including grain protein content, amino acid composition, and fat content, influence the nutritional quality of rice, with grain protein content being particularly significant. This study conducted screening and evaluation of crude protein content across 200 recombinant inbred lines of rice, derived from an inter-specific cross between BPT5204 and HPR14 parents. Results indicated a range of total crude protein content from 14.99 (mg/g) to 28.11 (mg/g) among samples. Grouping analysis based on available crude protein content categorized the lines into three major groups: approximately 3% (7 lines) exhibited significantly higher protein content (>25mg/g), 59% (119 lines) displayed moderate content (20 mg/g to 25 mg/g), and 38% (76 lines) showed low protein content (<20 mg/g). The identification of high protein content genotypes in this study holds potential for mapping key genomic regions associated with protein content in rice.