Steroidal Saponins Isolated and Purified from Leaves Extract of Asparagus racemosus (Family – Asparagaceae) and its UV, IR, NMR and Mass Spectrum Analysis

Asparagus racemosus is an important medicinal plant Its medicinal usages has been reported in Indian and British pharmacopoeias. Herbs made by the Asparagus racemosus are highly effective in problems which related with female reproductive system and other diseases. The Fresh plant leaves were collected from Govt. Science & Commerce College, Benazir, Bhopal (M.P.) in July-September 2012. Qualitative preliminary phytochemical analysis was performed on the crude extract for the presence of bioactive compounds. The pure compound isolated to the Column chromatography and purified this compound by Thin Layer Chromatography with suitable solvent systems and visualizing reagents.

Structure elucidation of pure compound has been accomplished through the extensive use of UV-Visible, IR, 1HNMR and Mass spectroscopy. Isolated compound was gave a positive test Liebermann-Burchard test for steroidal saponin and the compound obtained as dark brownish amorphous powder as a single spot with 0.37 Rf value. Phytochemistry and spectral analysis showed the presence of steroidal saponin in Asparagus racemosus leaves extract. It is strongly believed that the phytochemical and various biological properties of the extracts might provide detailed evidence for the use of this plant in different medicines.