REDDY, SRINIVASA and RAMANNA, LOKESH and JOSEPH, JIBIN and THOMAS, LICTO and MUKHOPADHYAY, ARINDAM and THANGAM, SARAL (2015) DEVELOPMENT AND VALIDATION OF A LIQUID CHROMATOGRAPHY – MASS SPECTROMETRY METHOD FOR ESTIMATION OF PRAZIQUANTEL IN HUMAN PLASMA. Journal of International Research in Medical and Pharmaceutical Sciences, 6 (1). pp. 1-9.
Full text not available from this repository.Abstract
Schistosomiasis is one of the most frequently occurring tropical diseases in the world. Praziquantel is the drug of choice worldwide for treatment and control of schistosomiasis. A highly sensitive LC-MS/MS for quantification of praziquantel was developed and validated. Chromatographic separations were achieved using an isocratic binary mobile phase [2 mM ammonium acetate (containing 0.05% formic acid): Acetonitrile: 45:55v/v] at a flow rate of 1.0 mL/min on a C18 column (100 x 4.6 mm i.d) maintained at 50°C and estimated in API 4000 Mass spectrometer in the positive atmospheric pressure ionization mode by following multiple reactions monitoring (MRM) transitions for parent and daughter ions. Deuterated praziquantel was used as an internal standard. A linear calibration plot was achieved for in the concentration ranges of 1.012 ng/ml to 751.552 ng/ml. The mean percentage of recovery was 87.34 with mean % CV of 5.19. It is a fully validated method and can successfully be applied for estimation of praziquantel during biostudies.
| Item Type: | Article |
|---|---|
| Subjects: | Universal Eprints > Medical Science |
| Depositing User: | Managing Editor |
| Date Deposited: | 27 Nov 2023 03:34 |
| Last Modified: | 27 Nov 2023 03:34 |
| URI: | http://journal.article2publish.com/id/eprint/3272 |
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