Role of Biochemical Parameter in Synthetic Biomolecular Network of Escherichia coli

The role of biochemical parameters (like rate constant of expression of repressor) to determine the nature of stability for manipulating gene regulation in Escherichia coli is well established with toggle switch model. With the higher order cooperativity of repression of promoters, the robustness of the system increases which in turns help to achieve the bistability in case of weaker promoter. The histogram represents the sudden switching from low to high state. The release of protein concentration with time has studied in two approaches. Effect of noise is studied and observed that noise changes with system size. Noise strength is also measured.