Standardization of In-vitro Regeneration Protocol in Gerbera jamesonii Bolus Ex Hooker F.

Introduction: The gerbera belong to the family Asteraceae is the chief cut flowers and ranks among the top ten cut flowers in the universe. For commercial propgation of this plant species, planting material is required on large-scale which requires the employment of plant tissue culture techniques for massive in vitro propagation.

Study Objectives: In this investigation, an effort was made to compute optimal concentration of plant growth regulators added in culture medium and optimize other physical factors exhibiting higher in vitro response by culturing mature embryo in vitro.

Results: Nutrient media MS3D.5B (MS + 3.0 mgl-1 2, 4 D + 0.5 mgl-1BA + 30.0 gl-1 sucrose + 7.5 gl-1 agar powder) evidenced more appropriate for callus initiation. Inoculation media MS2N.5iP/MS3N.5ip (MS + 2.0/3.0mg l-1 NAA + 0.5 mgl-12-ip + 30.0 gl-1 sucrose + 7.5 gl-1 agar) displayed higher in vitro response i.e., numbers of shoot proliferating explants and numbers of shoot (s) per explant. While, shoot of higher length was recovered on culture medium MSB/MS2B (MS + 2.0/3.0 mgl-1BA + 30.0 gl-1 sucrose + 7.5 gl-1 agar). Enhanced in vitro rooting response (root proliferating efficiency, numbers of roots and root of higher length) were documented on rooting medium MS.1IB (MS + 0.1 mgl-1IBA + 15.0gl-1 sucrose + 7.5 g l-1 agar). The regenerants were transferred to pots and hardened in Environmental Growth Cabinet and net House and subsequently shifted to field conditions efficaciously. Phenotypic normal plants were acquired.