Study the Effect of Growth Regulators on Micropropagation of Gloriosa superba L. from Seeds and Their Acclimatization

Background: G. superb L. is an important medicinal plant of Ayurvedic system of medicine. It is distributed naturally widely throughout the tropics, and worldwide as a pot plant. Due the poor germination and low viability of seeds plant required more time for growing. As seed germination is poor and vegetative propagation is slow, The methods of rapid micropropagation are required. Procedure for seed germination and micropropagation were established.
Results: Fresh and healthy seeds were cultured on Murashige & Skoog medium supplemented with different concentration of BAP and GA3. Best seed germination was observed at 1.0 mg/l BAP and 0.5 mg/l GA3 at minimum time of 49 days. MS medium supplemented with 1.5 mg/l BAP+ 0.5 mg/l IAA and 0.5 mg/l kinetin recorded the highest response of shoot multiplication (3.0±0.3) with best shoot length (3.2±0.24 cm). The regenerated shoots were cultured on MS medium. 1.5 IBA and 0.5 NAA mg/l was found to be the best concentration for maximum percent of root initiation. The rooted plantlets were successfully transferred into plastic cups containing sand, peat and soil in the ratio of 1:1:1 and subsequently established in the greenhouse. Thereafter they were reared for three weeks. The survival rate of regenerants was found to be 60%.
Conclusion: According to the observations suitable combination of cytokinin and GA3 proved to be effective in the case of seed germination. In shoot cultures of G. superb L. BAP proved to be the effective cytokinin too.