Disentanglement of Staphylococcus aureus in Chicken by Enzyme-Linked Fluorescent Assay

This study is aimed to detect Staphylococcus aureus (S. aureus) in the chicken using conventional methods (biochemical tests), latex agglutination, and Vitek Immuno Diagnostic Assay System (VIDAS) system. These methods are considered as the modern methods to proven technology for the detection of foodborne pathogens. In this study, 100 samples were collected from the supermarkets in Baghdad city. The isolated colonies were picked up by Chromogenic and Baird Parker Medium and transferred onto a fresh medium to ensure purity based on phenotypic characteristics, biochemical tests, AIP® Staph kit, and the detection of enterotoxin using VIDAS SETII. The results were showed that all samples were decontaminated by S. aureus and toxic. Toxin A, B, and C has high-frequency of 0.25-2.00 ng g-1, 1.00 ng g-1, and 0.25 ng g-1, respectively. The toxin (A) was found to be more than (104 – 105) CFU ml-1.