Anti-bacterial and in vitro Anti-diabetic Potential of Novel Isoxazole Derivatives

Aim: To synthesize novel Isoxazole derivatives, characterize them and subject for screening anti-bacterial action and in vitro anti-diabetic activity.

Methodology: Chalcones were prepared by the reaction of aromatic aldehydes with aromatic ketones in aqueous alcoholic alkaline medium. Then these were made to react with hydroxylamine hydrochloride and sodium acetate to prepare title compounds. The prepared isoxazole compounds were subjected to in vitro anti-diabetic screening by yeast and enzymatic method. All compounds were screened for antibacterial action by disc diffusion method.

Results: The structure of the synthesized compounds were confirmed by IR, NMR spectral datas and screened for anti-bacterial, and anti-diabetic activities. Most effective antibacterial one possessed chlorine in the Phenyl ring attached at 5-C of isoxazole and have NH2 substitution in phenyl ring attached at 3-C of isoxazole. Compounds with significant in vitro anti-diabetic action by studying the glucose uptake by yeast cell method are with Br/NO2 substituted forR2/R3 in phenyl ring when R’2 is OH/NH2.

Conclusion: Presence of halogenated aromatic ring at 5-C and amine substituted phenyl ring at 3-Cof Isoxazole exhibited moderate anti-bacterial activity. In the anti-diabetic study halogenated or nitrated phenyl ring at 5-C and hydroxyl/amine substituted phenyl ring at 3-C of isoxazole exhibited anti-diabetic action.