Response Surface Optimization of Extraction of Polyphenols and Carotenoids from Sesbania grandiflora Leaves with Ethanol-water System

Aim: Response surface methodology was employed to optimize the extraction parameters for the extraction of total phenolics and carotenoids from leaves of Sesbania grandiflora with ethanol-water based system.

Method: The effects of solvent concentration (30-100%), extraction temperature (30-60°C) and extraction time (30-90 min) on the recovery of total phenolics and carotenoids were investigated. Results and Discussion: The constructed models were adequate to explain the behavior of the extraction system and predict the responses, total phenolics (R2 = 88.53%) and carotenoid (R2 = 90.60) contents. The optimum extraction conditions of ethanol concentration, extraction temperature and extraction time for phenolics, were 46.6%, 70.2°C, and 110.5 min and for carotenoids, the optimum parameters were 100%, 70.2°C and 110.5 min, respectively. The optimal predicted contents for total phenolics (7.74 mg Gallic Acid Equivalent (GAE)/ g DW) and carotenoids (4.32 mg/g DW) values in the extracts were agreed with the experimental values obtained with optimum extraction conditions for each response.